rabbit polyclonal antibody against p53 Search Results


rabbit polyclonal antibody against p53  (Abiocode Inc)
90
Abiocode Inc rabbit polyclonal antibody against p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Rabbit Polyclonal Antibody Against P53, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against p53/product/Abiocode Inc
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against p53 - by Bioz Stars, 2026-03
90/100 stars
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primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53  (Novocastra)
90
Novocastra primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Primary Rabbit Polyclonal Antibody Against Human P53 That Cross Reacts With Feline P53, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53/product/Novocastra
Average 90 stars, based on 1 article reviews
primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit polyclonal antibodies against p53 201  (Wanleibio)
90
Wanleibio rabbit polyclonal antibodies against p53 201
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Rabbit Polyclonal Antibodies Against P53 201, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies against p53 201/product/Wanleibio
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against p53 201 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Establishment of p53 stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of p53 protein level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cell-mediated suppression of hypertrophic scarring is p53 dependent in a rabbit ear model

doi: 10.1186/scrt526

Figure Lengend Snippet: Establishment of p53 stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of p53 protein level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.

Article Snippet: Rabbit p53 shRNA lentiviral particles, control shRNA lentiviral particles and rabbit polyclonal antibody against p53 were purchased from Abiocode Bio-Technology (Shanghai, China).

Techniques: Generated, Transduction, shRNA, Clone Assay, Selection, Western Blot, MTS Assay, Staining, Flow Cytometry, Negative Control